 |
 |
 |
 |
 |
I'm trying to quantify the amount of complement factor H deposited on
Neisseria gonorrhoeae by FACS. I'm using a BD FACScan machine. When
acquiring the data I have the FSC set at E-1 (PMT is at 868) and I'm
noticing that I'm getting non-specific background noise that will not go
away even when water is running thru the machine. Do you have any
suggestions about what settings (FSC/SSC) I should be using to analyze
bugs with this machine and any thoughts on the background noise.
Thanks in advance for any help.
Scott Simpson
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web