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I saw a few postings on tunnel staining and Annexin V staining for
detection of cells undergoing apoptosis. I was told that Annexin V
labeling actully could detect apoptosis before actual DNA fragmentation
(detected by Tunnel assay) occured. Do this make Annexin V labeling
better method for detecting apoptosis ? Obviously, Annexin V staining is
much easier, thus more convenient than Tunnel, but I wonder if Annexin V
expression is better marker than DNA fragmentaion for the apoptotic
cells.
Aki Hoji (akhst7+@pitt.edu)
University of Pittsburgh
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web