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Frederick Garbrecht
Thomas Jefferson University
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> From: Walter Sharp <102675.320@compuserve.com>
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: Heat stability
> Date: Friday, October 11, 1996 12:49 AM
>
>
> The investigator mentioned by Jill Martin is quite right to refuse the
antibody.
>
> We here (Oman in the Persian Gulf) have had some experience with
overheating,
> given that the temp. can easily exceed 120 deg.
> We had a case recently where two antibodies were shipped by a company (I
wonder
> if it's the same one?) without ice.
> One was totally "dead" (CD55), one working but, to my mind, weakly so
(CD59).
> They were returned to sender who gave the same spiel about "we've never
had a
> problem before".
> The dead one was replaced, the weak one was sent back to us since it was
> "working".
> Surprise, surprise - it was totally dead by the time it reached us the
second
> time, only a few weeks later.
>
> The upshot is, in my view, the least one can expect is a shorter shelf
life of
> the more robust antibodies and a quick death for the fragile ones.
>
> I used to work for Coulter here in the middle east as their "specialist"
and
> there were many occasions where my troublshooting of customer problems
showed up
> inappropriate storage at site of use and/or transport of delicate "juice"
at
> ambient (120 + deg) temperatures.
>
> Wal Sharp
> Sultan Qaboos University
> Oman.
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web