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I am looking for references and/or advice on the following:
Staining live, unfixed bacteria with Hoechst 33342 (or another DNA stain =
with similar spectral characteristics). I tried loading bacteria with H =
33342 but they did not take up the dye. Do I need to use EDTA or some =
other agent to get the H 33342 into the bacteria?
Does anyone have any tips or good references in general on sorting =
bacteria using a flow cytometer.
Thanks in advance,
Tom Knapp
Aurora Biosciences Corporation
11149 N. Torrey Pines Road
La Jolla, California 92037 USA
email "knappt@aurorabio.com"
Web Site "http://www.aurorabio.com"
(619) 452-5000 ex 125
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web