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We here (Oman in the Persian Gulf) have had some experience with overheating,
given that the temp. can easily exceed 120 deg.
We had a case recently where two antibodies were shipped by a company (I wonder
if it's the same one?) without ice.
One was totally "dead" (CD55), one working but, to my mind, weakly so (CD59).
They were returned to sender who gave the same spiel about "we've never had a
problem before".
The dead one was replaced, the weak one was sent back to us since it was
"working".
Surprise, surprise - it was totally dead by the time it reached us the second
time, only a few weeks later.
The upshot is, in my view, the least one can expect is a shorter shelf life of
the more robust antibodies and a quick death for the fragile ones.
I used to work for Coulter here in the middle east as their "specialist" and
there were many occasions where my troublshooting of customer problems showed up
inappropriate storage at site of use and/or transport of delicate "juice" at
ambient (120 + deg) temperatures.
Wal Sharp
Sultan Qaboos University
Oman.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web