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On September 24, Robin Barclay wrote:
>Does anyone know whether phenol red, the common pH indicator in tissue >culture
medium, has any fluorescent properties when associated with >cells or any
quenching properties for other fluorochromes?
------------------------------Reply-------------------------------------
Phenol Red which is the indicator used in RPMI-1640 as well as other culture
media is excited by 488 nm and emits over a wide spectrum. Cells that have not
been adequately washed of tissue culture media before being passed through the
cytometer will fluoresce and be picked up by the fluorescent detectors. Negative
cells will appear to be double, triple or quadruple positive depending on the
number of fluorescent detectors you are using.
As for quenching, perhaps someone can address that question. The fluorescence
generated from the phenol red alone will affect your results and the ability to
differentiate populations with different staining intensities.
Steven Merlin
University of Bern FACS Core Facility
Bern, Switzerland
merlin@patho.unibe.ch
101234.534@compuserve.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web