PE/Cy5 on a FACS Calibur

Calman Prussin (CPRUSSIN@atlas.niaid.nih.gov)
Mon, 23 Sep 1996 20:22:06 -0400

An associate has asked me a third hand question regarding the utility of
using PE/Cy5 conjugates on a FACScaliber in 3 color applications.
Allegedly, the investigator was told by the BD salesperson that PE/Cy5
conjugates (Cychrome, Tricolor, Quantum Red, etc.) do not work on a
Calibur and that they had to use PerCP. That seems highly unlikely.
Perhaps what the salesperson said, or meant to say was "PE/Cy5
conjugates will not work in 4 color/ 2 laser applications on the
Calibur".

Questions for the group and for BD lurkers in particular:

1. Do PE/Cy5 conjugates work any better or worse on a Calibur than on a
FACSCAN in 3 color/ 1 laser applications.

2. What is the present status of 4 color work on the Calibur? Any plans
for cross-beam compensation to allow simultaneous use of FITC, PE,
PE/Cy5 and APC?

Thanks,

Calman Prussin

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