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We usually have folks bring loaded cells on ice, then warm to 37
before calcium analysis. This works on most of the cell types we see,
although I can't remember if this ever caused trouble with PBL's.
I think this temperature thing is cell type specific- i remember
seeing Jurkatt cells flux on ice!!
Peter
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Peter A. Lopez (617)632-3179 (voice)
Dana Farber Cancer Institute (617)632-3139 (voice)
Core Flow Cytometry Facility NO FAX!
44 Binney St. Room J-312
Boston,MA 02115 PLOPEZ@sorter.dfci.harvard.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web