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> TIP-OF-THE-DAY:
>
> We recently moved solid tumor DNA cell-cycle analysis from an EPICS-
> 753 to our new ELITE. However, using TruPloid drift analysis
> software, we detected a large upward shift in fluorescence intensity
> during the first few minutes (2-4) of each new sample acquisition on
> the ELITE. The fluorescence eventually would stabilize.
We have experienced precisely this problem with the ELITE when using 7AAD
instead of PI for cell cycle analysis. We got round it by isng time as a
parameter and gating out events before the machine stabilized. Your
solution seems a lot nicer, I will let you know if it works for 7AAD as
well.
Mike Salmon
Birmingham University, UK
E-mail: salmonm@rheuma.bham.ac.uk
Tel: 44 (0)21 414 6780
FAX: 44 (0)21 414 6794
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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