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emits in the high 400's (465-490)."
Jeff Wang makes excellent beads for this purpose. His RFP-30-5A product is a
single bead that has fluorochromes that excite in uv and fluoresce into violet
to blue (ca. 430 to 490nm); they also excite at 488nm and fluoresce from the
green to the red (530 to 680nm). We use them for instrument alignment when
doing 5 color. The intensity of this single bead is typical of many labeled
cells.
There is also a mix of beads (RCP-30-5) that is comprised of 6 populations with
vary amounts of the fluorochromes. This provides a blank and 5 labeled beads
that span the entire histogram for green to red emissions, and about half the
half the histogram for blue and violet.
The forward scatter cv's are about 1.5% and linear green fluorescences are
mostly below 2% as I recall. Particle stability is excellent: at least 3 years
would be a good guess if refrigerated.
Jeff Wang is at:
Spherotech, Inc.
1840 Industrial Dr. Suite 270
Libertyville IL 60048
tel. 708-680-8922
fax. 708-680-8927
Dave
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web