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This method, however, is clearly not applicable for day-to-day running and
adjustment of the cytometer. We have had very good results with beads from
Spherotech (1840 Industrial Dr. #270, Libertyville, IL 60048). They make beads
with multiple dyes in them that excite from the UV to the red and are quite
stable over time. We currently use their #NV4C1C18-RM20, which has good signal
and CV in the UV and matches your output spectral reqirements.
-Marty Bigos
Stanford Shared FACS Facility
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web