Some help for a first time user
Markl Kido - the Rasta-Ganja BuddHA (MARK@git.uct.ac.za)
3 Nov 93 09:06:35 SAST-2
I am isolating colonic epithelium from biopsies for flow cytometry.
I am using a simple aspiration technique to help disaggregate the
cells into a partial single cell suspension (5-10% clumping), and
then filter this to remove clumps. However I am concerned about the
amount of debris these 2 procedures generate. Anything up to
50% of the material under the microscope can be cell fragments. Does
anyone have any ideas about cleaning this up? or alternatively a
system which decreases debris production? I would be most grateful
for some advice.
Thanks in advance
Mark Kidd
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
