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On Wed, 13 Oct 1993 kharkins@iastate.edu wrote:
>
> In reference to the lacZ question, my experience has been that the FDG or
> lacZ kits are to be used with cells in the "viable" state, and that these
> substrates are sensitive to the slightest perturbation of the cells which in
> turn causes them to leak out. Does anyone know if the cleaved product readily
> remains inside the cell once cleaved, should you then wish to "fix" the cells
> for DNA/cell cycle staining? As Jeff stated, we are interested in using
> beta-gal as a marker to distinguish one cell type from another within a mixed
> culture, so that we can then in turn identify the individual cell cycle
> distributions of each cell lineage.
>
> Kristi Harkins
> kharkins@iastate.edu
>
>
>
>
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web