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In message Fri, 20 Aug 1993 16:48:59 PST,
Mike_Lance@bdis.com (Mike Lance) writes:
> Subject: Time:4:38 PM
> OFFICE MEMO Ratio on FACS Vantage/FACStar+ Date:8/20/93
>
> The FACS Vantage and the FACStarPLUS are both highly adaptable. However,
> ratioing the delayed signals between two lasers is not possible on either
> system. The suggestion of using the FL2 signal cable to "trick" the
> instrument will result in signals from two events being ratio'ed, not two
> parameters from a single cell being ratio'ed which is probably what you
> want. This is because of the timing of when the signal is ratio'ed, which
> occurs right after the peak. Thus, the ratio is determined right after the
> the particle goes through the first laser (on an FL1/FL2 ratio), before it
> is even seen by the second laser! So if you "trick" the system this way,
> the ratio is actually the ratio of an FL1/"FL2" event that occurred at the
> same time as an actual "FL4" event.
>
> Angela Schultz
> Product Manager, FACS Vantage
{---------------------------------------------------------------------}
Douglas Smoot
Naval Medical Research Institute, Code O6
Bethesda, MD 20889-5607
(301) 295-1121
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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