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Anyway, the technique seems to work fine giving a nice sharp G0/G1 spike, until
we run a large number of events. This is essential for trying to characterise
the activated lymphocytes in our clinical samples; but the problem is, there
is a shift to the right of the G0/G1 peak after about 18,000 events. We are
using a Coulter ELITE, the 7AAD is excited with an Argon laser, and detected
in PMT4 with a 640LP filter.
Has anyone encountered this before? If not, any bright ideas would be welcome.
Best Wishes & Have Fun
Mike Salmon
Email to salmonm@rheuma.bham.ac.uk Voice: 021-414-6781
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web