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Corn pollen (from PolySciences), about 90um in diameter, was used as
a large test particle. The roughly 60us pulse width for pollen was
as expected (beam height is assumed to be 20um at focus). However,
there were substantial problems with clogging, hence, a sample tubing
with larger internal diameter (and about the same outside diameter)
is needed. Any reliable sources?
Further, sample settling is, of course, a problem and the informal
documentation recommends against using sample agitate while sorting,
but suggests the addition xanthan gum at 0.1% concentration. Any
experience with this vis a vis, fluidics effects (viscosity of the
sample stream will go up), sorting efficacy, sterility of sorted
cells?
Any other gems of wisdom in sorting large particles on the FACStar?
Dave Coder
Cell Analysis Facility
Univ. Washington
Internet: dcoder@u.washington.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web