Good CV's for DNA Histograms

Harbor Branch Oceanographic Institution (harbolon@class.org)
Fri, 30 Apr 1993 18:38:11 -0700 (PDT)

Could anyone tell me the trick to get good C.V.s for DNA histograms. The
cell line I am using is the T-hybridoma DO11.10. I stain with P.I. using
Vindelov's procedure. I always get G1 peaks with C.V.s of 4.0 to 5.0. I
want them tighter!! I also notice that the S phase is "fat" and little to
no G2/M peak. Any suggestions? (I use argon excitation @ 30 mW and
emission @ 690 nM.

Thanks!

Ross E. Longley, Ph.D.
Division of Biomedical Marine Research
Harbor Branch Oceanographic Institution, Inc.
Phone (407) 465-2400, Ext 486
FAX (407) 465-1523
INTERNET harbolon@class.org

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