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We are preparing for a cytometry run with the C2 muscle line that, in
our experience, adheres to itself after separation in about 10
minutes (nice big clumps of cells that won't go through the probe).
I've run sticky cells before with EDTA-PBS solutions but my user
wants to use heparin sulfate. Does anyone know if the stuff
autofluoresces?
- ivan
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Ivan Shaw "The Angel of Death"
Neuroimmunology Unit
Montreal Neurological Institute
Montreal, Canada H3A 2B4
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Internet e-mail: Ivan@MNI.LAN.McGill.CA
Telephone: (514) 398-8534
FAX (514) 398-7371
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web