Hepatocytes

hilliard@zookeeper.zoo.uga.edu
Wed, 14 Apr 93 15:41:42 EST

I have a user who's advisor just told her "find a protocol for DNA
analysis of liver cells in fish--we'll be collecting samples the first
week of May". Vindelov and Christensen's procedure (in Darzynkiewicz and
Crissman) seems too easy--does anyone have any caveats? How should they
process samples--freeze a section dry at -70 and needle aspirate in the
lab? Aspirate from fresh and rinse into buffer to freeze? How will they
avoid lots of RBC's? Any warnings will be helpful--we'll be working out
the kinks soon (hopefully!).

Steve
Steve Hilliard : "Be good, and you will be
Cell Analysis Facility, Univ. of GA : lonesome."
email: hilliard@zookeeper.zoo.uga.edu :
voice: (706)542-9474 : I brew the beer I drink!

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu