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The problem I've seen when using PI and PE is that some cells will take
up not enough PI to get gated out , and these cells may look like dull
PE positives due to the small amount of PI taken up. This has shown up
in a number of situations-
1- Cells in too high a PI concentration and/or in it for too long.
2- COS cells.
Some adjustment of PI concentrations may help.
Peter Lopez
Dana Farber CAncer Institute
Plopez@sorter.dfci.harvard.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web