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This is the way I do it and it works nice for me. If someone has a different
opinion about how to do it please don=B4t hesitate to correct me.
Micael Granstrom
FOA ABC-skydd
S-901 82 Umea
Sweden
e-mail mgranstrom@ume.foa.se
______________________________ Reply Separator=
_________________________________
Subject: Ethanol fixation
Author: "Bruce S. Hass" <BHASS@nctr.fda.gov> at Internet
Date: 9/10/96 10:50 PM
Hi,
I am interested in staining cells with Bodipy by the method of Gocze=20
and Freeman (Cytometry 17:151-158 1994), which calls for ethanol=20
fixed cells. Unfortunately the paper does not give details about the=20
fixation procedure. Is ethanol fixation so simple, obvious, or common?=20
Any suggestions on how to do it would be appreciated. Thanks.
=20
Bruce Hass
National Center for Toxicological Research=20
Jefferson AR 72079
(501) 543 7365
(501) 543 7745 (fax)
bhass@nctr.fda.gov=20
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web