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Running a FACStar Plus with Dye laser at about 605nm;
I recently got a better pump laser, so was able to turn up the wick
on dye laser to about 150mW (I think!).
Wonderful improvement in both APC and Texas Red :-)
But now we are getting PKH-26 breaking into the APC channel! :-(
Strangely, not so much into the Texas Red channel... ;~}
I don't have a good picture of spectra for PKH-26, don't know if it is
absorbing at 605, but it does appear to have a long emission tail.
Any experience like this?
Ideas &/or info greatly appreciated.
Much Thanks, Joseph.
Joseph Webster (O.I.C. Flow Cytometry)
Centenary Institute of cancer Medicine & Cell Biology
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-9565-6110 Fax: 61-2-9565-6101
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web