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Dean Hewish recommends adding sodium hypochlorite to ethidium bromide
solutions. However, reference to Sambrook Fritch and Maniatis
(Molecular Cloning, a lab manual) turned up the following:
"Treatment of dilute solutions of ethidium bromide with hypochlorite
is not recommended as a method of decontamination. Such treatment
.... converts the dye into a compound that is mutagenic ....
Quillardet P and Hofnung M Trends Genetics 4:89 (1988).
Draw your own conclusions,
Nigel Blackhall
University of Nottingham.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web