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Forward scatter, however, is even more complex. It's related to refractive
index as well as to size of the particle. And, of more relevance, it is
proportional to cross-sectional area of the particle (NOT to diameter). The
larger the cross-sectional area of the particle, the more light passes through
it and gets refracted around the obscuration bar to reach the photodector. So,
even if refractive indices are constant from particle to particle and even if
the particles are larger than the laser beam (both highly questionable), you
would not expect a straight line relationship between FSC and pulse width, but
rather a curve.
Let someone else take over from here!
Alice L. Givan
Englert Cell Analysis Laboratory
Dartmouth Medical School
Lebanon, New Hampshire
NH 03756 USA
tel 603-650-7661
fax 603-650-6130
e-mail givan@dartmouth.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web