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In my opinion it would be easiest for you to use fluorescent
microspheres already coated with some protein. If the protein of
interest for your user is an antibody, you could probably use protein
A or protein G microspheres. Alternatively, you could biotinylate
the protein and use it in conjunction with streptavidin coated
FluoSpheres.
However, you should be careful in adding the streptavidin
FluoSpheres dropwise to a dilute solution of the biotinylated protein.
Also, in my experience the 40nm streptavidin FluoSpheres work well
for cell surface receptors (data presented at ISAC in Rimini in April
1996).
______________________________________________________________
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Mahesh K. Bhalgat, Ph.D. M M M M BBBBBBBB
Molecular Probes, Inc. M M M M B B
Eugene OREGON 97402 M M M B B
M M BBBBBBBBB
Phone: (541) 465-8300 M M B B
Fax: (541) 344 6504 M M B B
E-mail: mahesh@probes.com M M BBBBBBBB
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web