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Margaret North and John Farrant, Royal Free Hospital School of Medicine, London,
have supplied material for my CD-ROM ('Data Analysis in Flow Cytometry - a
dynamic approach' available from Phoenix Flow Sytems) showing staining of
mononuclear cells from human peripheral blood. The cells were incubated for 12 h
either with monensin (unstimulated) or with phorbol myristate acetate (PMA),
ionomycin and monensin (stimulated). They stained for IFN-gamma, TFN-alpha and
IL-2. Their listed data files are on the CD-ROM. The accompanying book gives
details of the method.
Michael Ormerod
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web