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We wish to determine ploidy of industrial yeast (up to 6n) from
listmode FACScan data. We are using 7-AAD at low concentration so
aren't too worried about rRNA. We grow the cells to minimise
mitochondrial interference. We therefore only need a program to take
account of: cell cycle stage/cells with attached buds/doublets.
Are you familiar with a good program for this that we can use for free (or
cheap!)
Thanks for your help,
Dan
_______________________________________________
Dr Dan Deere (ddeere@rna.bio.mq.edu.au)
Research Fellow
Australian Environmental Flow Cytometry Group
School of Biological Sciences,
Macquarie University,
Sydney,
Australia NSW 2109.
Tel- +61 2 850 8150/8238 or 8685510
Fax- +61 2 850 8245
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web