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i am working on monitoring sebocyte lipogensis and recently started using
the ratio of FL2/FL3 fluorescence of nile red to monitor the extent of
staining of cytoplasmic lipid droplets and membranes/other irrelevant
cell material.
the interesting thing is when i plot this ratio as a function of forward
scattering, i get a nice horizontal linear ridge around FL2/FL3 of 48 with
peaks at different FSC points. what do you think may be causing this? i am
willing to fax a copy of the plot if somebody thinks that they may be able
to offer ideas.
thanks very much,
alex.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web