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I have an investigator who has been obtaining peritoneal macrophages from
mice by lavage, culturing them overnight with TNF, and then looking by flow
for Ia expression. We weren't getting much expression so I had him stain
with PI at the same time. It turns out that the vast majority of the
cells are dead. He has tried culturing in suspension or letting the cells
adhere and using cold to detach them. Does anyone have a method of
detaching that is easier on the cells and still leaves surface markers
intact? Any advice is greatly appreciated.
Carol
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Carol Oxford _| _| _|_|_|_| _|_|_|
Medical Pathology Flow Cytometry Lab _| _| _| _| _|
University of California _| _| _| _| _|
Davis, California 95616 _| _| _| _| _|
(916) 752-7205 _| _| _| _| _|
(916) 752-4548 fax _|_|_|_| _|_|_|_| _|_|_|
cloxford@ucdavis.edu
University of California, Davis
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web