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The monocytes contamination in a lymphoid gate is not resolved with a
CD45vSS, due to the fact that the altered monocytes have both the SS and
CD45 very similar to the lymphocytes; as opposed to granulocytic
contamination that can be solved by SSvCD45 gating.
If the lymphoid gate in FSvSS is contaminated by monocytes, you should
add a monocytoid line marker(CD14) to remove these cells.In any case a
2-3% of contamination is not a high value. I recommend you to do a
lymphoid gate in order to separate better the different populations from
a fresh sample.
The decrease problem expression can be solved titering the antibodies
and/or scanning the voltages of your cytometer.
The ideal panel to determine kappa/lambda is a matter of each particular
lab, but I strongly recommend you kappa/lambda/CD19 PerCP in the same
tube. We use kappa/lambda from Dako and CD19 from Becton Dickinson, and
we have great results.
Marco A. Fernández
e-Mail: reference@cinet.fcr.es
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web