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I don't know about measuring fetal RBCs in maternal blood, but I was
surprised when we tried to run our stem cell panel on umbilical cord
blood and found the RBCs very difficult to lyse by conventional means:
NH4CL, R&D Systems kit, etc. Does this add any light to the subject?
In addition, and along the lines of studying fetal/cord/maternal
blood, I would like to know:
(1) Why cord blood RBCs behave different from adult peripheral blood
and fetal blood under the standard lysis conditions.
(2) Does anybody know of a way to lyse cord RBCs and leave the WBC
intact?
Thanks
Steve Micko
Emory Hospital
Atlanta
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web