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I've found it works for surface antigens on BAL cells for 48
hours.....longer than that is not described but you could try it in your lab
first and if it worked it would save you having to bring antibodies to
wherever you're going and label them there.
Here is one of the references:
Openshaw P; Murphy EE; Hosken NA; Maino V; Davis K; Murphy K; O'Garra A.
J Exp Med 1995 Nov 1;182(5):1357-67
What they don't tell you in these papers is that when you take your cells
out of storage to label them, check your sample with a fluorescent
microscope as crystals tend to accumulate and you need to wash them off.
I hope this works
Dearbhaile O' Donnell
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web