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I've found it works for membrane antigens for 48 hours.....longer than
that is not described but you could try it in your lab first and if it
worked it would save you having to bring antibodies to wherever you're going
and label them there.
Here is one of the references:
Openshaw P; Murphy EE; Hosken NA; Maino V; Davis K; Murphy K; O'Garra A.
J Exp Med 1995 Nov 1;182(5):1357-67
What they don't tell you in these papers is that when you take your cells
out of storage to label them, check your sample with a fluorescent
microscope as crystals tend to accumulate and you need to wash them off.
I hope this works
Dearbhaile O' Donnell
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web