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______________________________ Reply Separator _________________________________
Subject: Re: fixing and storing cells
Author: Alice.L.Givan@dartmouth.edu (Alice L. Givan) at biosmtp
Date: 6/13/96 11:13 AM
Hello Ruedi,
My general impression (but you should probably check it yourself) is that PE
and/or FITC-stained cells fixed in 1% paraformaldehyde (if kept cold and dark
after fixation) are very stable and can be read reproducibly after several
weeks or more. However, there are changes that occur rapidly upon fixation
(background fluorescence goes up a bit, PE fluorescence goes down a bit, some
cell types (eg granulocytes) change their scatter characteristics
significantly). Therefore, we always keep cells for at least 24 hours after
fixation before we analyze them -- to give time for these changes to stabilize.
Hope this helps.
Alice
Alice L. Givan
Englert Cell Analysis Laboratory
Dartmouth Medical School
Lebanon, New Hampshire
NH 03756 USA
tel 603-650-7661
fax 603-650-6130
e-mail givan@dartmouth.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web