2. This is a B cell question. What percentage of IgG and IgA positive B
cells can you detect in the mouse spleen, and lymph nodes? Do you need to
first gate on a B220 or CD19 positive population? What are good antibodies
to use for this purpose?
I would really appreciate any advice,
Dr. Meenakshi Roy
Dept. of Pathology, Stanford University
Stanford University/Foothill research center
R205, 4005 Miranda Avenue
Palo Alto, California, CA 94304
FAX: 415-858-3986
email: meena@macsun.stanford.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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![]() |
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web