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I personally prefer to use LDS-751 (I'm prejudiced) or fluorescein diacteate.
These reagents have less time dependence (note they stain apoptotic DIMMER, not
brighter), and have been very reliable in my hand. FDA has the advantage of
also indicating cells that have gone on to "secondary necrosis" or whatever you
prefer to call the result of the apoptotic process. FDA has the drawback of
using up your fluorescein channel.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web