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Pharmingen sell an antibody against mouse=20
dendritic cells (which I haven't tested yet). We=20
are using flow to identify dendritics from spleen=20
and epidermis. Note that they express M1/70=20
antigen (Mac-1) but to a lower extent than=20
macrophages. Also there is a small spleen=20
population that stains with RA3-6B2 (anti-B220) in=20
our hands (tubes..). If you can get rid of B cells=20
I guess that classII should be ok. Why not also=20
try class I?
Mikael
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web