acridine orange in FACScan

ZBIGNIEW DARZYNKIEWICZ (darzynk@nymc.edu)
Tue, 04 Jun 1996 08:55:43 -0500

We frequently measure the AO stained cells by FACScan, which
otherwise is used for analysis of the cells stained with PI and
immunofluorescence measurements, with no evidence of any negative
effects of AO on subsequent samples. Following AO, however, the
instrument is rinsed by running a bleaching agent for about 10 min.
Zbigniew Darzynkiewicz

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu