re:re: intracellular IL4 and IFNg

Mikael Vestberg (Mikael.Vestberg@inflam.lu.se)
Fri, 31 May 96 18:00:50 +0200

We have done analysis in mouse lymph node T cells=20
after in vitro culture. After 4 days or more of=20
culture w ConA and then a 6 hour PMA/ionomycin=20
treatment in the presence of monensin we see=20
abundant IFN gamma and some IL4. They are not=20
expressed in the same cells.

We use 50 ng/ml ConA and 50 ng/ml PMA and 1 ug/ml=20
ionomycin in the presence of 3uM Monensin.

Mikael

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu