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The trusty Ammonium cloride lysing solution is made up as follows:
Weigh out 8.29g NH4Cl plus 1.0g NaHCO3, dissolve in 1l distilled water and
adjust pH to 7.4.
The BD stuff has some secret formula which includes formaldehyde and
diethylene glycol. With the ammonium cloride lysing solution use 10 parts
lysing solution to 1 part blood. The white cells remaining are unfixed and
viable. Prepare yourself for many replies to this one!
By the way what was your embarassing problem?
Simon
Simon Monard
FACS Lab Manager
Aaron Diamond Center
455 1st Avenue
New York
NY 10016
ph (212) 340 3741
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web