Re: 7-AAD with fixation

Dave Coder (dave@nucleus.immunol.washington.edu)
Tue, 28 May 96 15:17:36 -0700

Several people have asked about the use of fixatives with 7-AAD detection of
non-viable cells as described by T. Fetterhoff et al. 1993. I've added a
basic protocol for this to the page that describes the general 7-AAD
procedure:

http://nucleus.immunol.washington.edu/Research_facilities/Apps/7aad.html

Click on the Fetterhoff reference for details.

Dave Coder

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David M. Coder, Ph.D.
Director, Cell Analysis Facility
http://nucleus.immunol.washington.edu/Research_facilities/cell_analysis.html

Editor, ISAC WWW Home Page
http://nucleus.immunol.washington.edu/ISAC.html

tel. 206-685-3014
fax. 206-543-3480
e-mail: dcoder@u.washington.edu

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu