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I remember that certain antigens (and certain antibodies) are killed by
paraformaldehyde fixing; I have not heard of flourochromes being affected
in the same way, but check it out anyway.
Sorry I can't find a list of affected stains, I'm sure those who work a
lot with fixed cells can provide some specific info.
The best test is simply to stain some cells, read them fresh, fix, then
repeat the reading.
Joseph.
-- Joseph Webster (O.I.C. Flow Cytometry) Centenary Institute of cancer Medicine & Cell Biology Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA. Ph: 61-2-565-6110 Fax: 61-2-565-6101
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web