RNA Stains

Howard Shapiro (hms@shapirolab.com)
Thu, 23 May 1996 20:39:34 -0400 (EDT)

Following up on Tom Frey's recent comment - I've tried blocking DNA
binding of thiazole orange and a couple of other cyanine nucleic acid stains
with Hoechst dye, and it doesn't seem to work (i.e., most of the dye
fluorescence remains after RNAse treatment, which is not the case with
pyronin. We keep hoping, though.
-Howard

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu