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Fra: owner-cyto-sendout
Til: Cytometry Mailing List
Emne: Kappa and Lambda
Dato: 16. mai 1996 09:57
Dear Flow-ers,
This is another basic kind of question ... but as usual I'm sure that I'm
not the only one who has trouble with this.
My staining for kappa/lambda expression is very disappointing - separation
of negative and positive populations is miserable and despite using CD45vSS
to selectively gate lymphoid cells, I still get 2-3% dual positive events
(using color backgating these dual positives appear to be occasional stray
granulocytes/monocytes.)
I'm wondering what the consensus is for clinical samples ie: kappa/lambda
dual labels or do people use kappa/CD19 and Lambda/CD19 (I doubt whether
this is necessary since I use CD45/SS as a primary gating strategy but I
could be wrong!!)
Also what brands of Kappa/Lambda antibodies have people found to be most
successful - I currently use BD.
As always, any suggestions greeted with many thanks.
Peter Chapple
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web