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We found propidium iodide not to be fixable in mammalian cells. It gets
trapped to some extent, however. PI is better retained in bacteria after
fixation. If you need a DNA dye to detect cells with permeable membranes,
that is fixable, you may try to use ethidium monoazide bromide. This dye is
UV-photocross-linkable. After a blast with UV, this dye should be bound
covalently to the DNA. I have myself not actually done this, therefore my
pre-empting statements. This dye is available from Molecular Probes, Inc.
(catalog # 1374); P.O. Box 22010; Eugene; OR 97402; tel: (541) 465-8300.
Martin Poot
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web