 |
 |
 |
 |
 |
I'm interested in performing one-dimensional flow karyotyping of
PHA-stimulated monkey leukocytes or EBV cell lines, using PI as the stain.
I currently have two references for the preparation of metaphase chromosomes:
Lundsteen C et. al., Cytometry 19:42 [1995]
Aten JA et. al., Histochemistry 87:359 [1987]
The first article uses colcemid to effect a metaphase block, but
the concentration is not specified. The older article uses a blocking
reagent I've never encountered, called vindesine. Is vindesine obsolete?
At least they tell me what concentration they used.
Do you have a favorite protocol?
Is there a comprehensive discussion of this technology available
anywhere -- say, a recent review or book chapter? Can I expect EBV-trans-
formed cell lines to show chromosomal alterations relative to the parental
cells?
Thanks in advance,
- John Ladasky
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web