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>Does anyone have experience with surface CD38/cytoplasmic kappa/lambda?
>I would like to find out the simplest procedure.
A recent paper in the BJH describes a method for plasma cell detection using
Ortho's Permeafix reagent.
They are using surface expression of B-B4 and cytoplasmic kappa-lambda.
H.C.T. van Zaanen et al. "A simple and sensitive method for determining plasma
cell isotype and monoclonality in bone marrow using flow cytometry". British
Journal of Haematology. 1995. 91. 55-59
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Len Brown 102554.2147@compuserve.com
Department of Haematology (968)515736 voice
Sultan Qaboos University (968)513419 fax
Al Khod, Code 123, Sultanate of Oman
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web