Re: CELLQUEST

Brian Sailer (sailer@telomere.lanl.gov)
Mon, 6 Nov 1995 07:00:40 -0700

>Hello everyone,
>
> I know there have been recent discussions on certain Cellquest bugs and I
>was wondering if anyone has ever experienced the following:
>
> We were aquiring a series of samples using Cellquest on a FACS Vantage. It
>took about 20 minutes to run the entire set. During aquisition, all
>samples had
>appropriate staining patterns when looking at FL4 vs FL5. However, when we
>went
>to analyze the samples, 3 out of the 15 samples had randomly changed all the
>parameters. FL4 and FL5 staining was lost, FSC and SSC even were lost. We
>discovered that FL4 was now in FL2 and FL5 was in FL4 and we could not
>find FSC
>or SSC. We can't understand this because all samples were run at the same time
>and it was not done in 3 consecutive samples but rather at random.
>

This also happened to us on our FacsCalibur. We discovered the problem was
not collecting all of the parameters during acquisition. Even if your
sample has not red fluorescence, for example, still collect this parameter.
The data file will have all zero's for this parameter, but your problem
should disappear.

Brian

Brian Sailer
Los Alamos National Laboratory
LS-1 M888
Los Alamos, NM 87545
Phone (505)-665-6479
Fax (505)-665-3024
email sailer@telomere.lanl.gov
"I like deadlines. They make a neat whooshing sound as they pass by."

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