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The premise is that lymphocyte subsets are evenly distributed throughout the
lymphocyte population as isolated by FS and SS - my experience is often the
opposite.
High degree of pleomorphism of CD 8's compared to CD4's in reactive pictures,
especially AIDS and I.M.
Conversely, my experience of normal bloods is that the CD3+/CD4+ population is
slightly more variable in size (i.e.FS) than normal CD3+/CD8+ and tightening of
the gate, or allowing the instrument to do it for you, exclude some of the
target population with consequent inaccuracy.
My opinion on tightening the lower end via CD45 can wait until later.
The reason I put this to the mailing list is that I am putting together a
"devil's advocate" lecture and would appreciate other views.
On the other hand I may just like a good argument !
Get back to me via the Mailing list or on <102675.320@compuserve.com>
Wal Sharp.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web