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Further to Ed's request about how people measure CD34+ cell numbers by flow.
As well as asking about whole blood vs mononuclear preps and whether to use
CD45 or not (and indeed which CD45 antibody) it would also be interesting to
know which clone of CD34 antibody people use (since there are at least three
distinct epitopes on the CD34 molecule).
Thanks
Ian Titley
Leukaemia Research Fund Centre
at the Institute of Cancer Research
237 Fulham Road
London
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web